For VHL genotyping, three primers were used to amplify wild type 244 bp PCR product, floxed 277 bp PCR product and deleted 161 bp PCR product VHL alleles; P1 5 GGCAAGCACTCTGTCACTGTTC 3, which is specific to genomic sequence upstream of the 5 loxP sequence; P2 5 GGCTATGGCATTGGGTGAGCTA 3, which is upstream of 3 loxP sequence; P3 5 CACCAGGACTCCTCTCGGACAT 3, which is downstream of 3 loxP sequence <a href=http://cialis.motorcycles>cialis generic tadalafil</a>